Human NNMT1 also known as Nicotinamide N-Methyltransferase, GenBank Accession No. NM_006169, a.a. 1-264(full length), with C-terminal His-tag, expressed in an E. coli expression system. MW=30 kDa.
host species: E. coli
Host species: E. coli
Specific activity: 57 pmol/min/ug
Immunogen Region
1-264 (full length)
Tag
C-terminal His-Tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, 145 mM Imidazole.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
10 mM Tris-HCl, pH 7.4, 0.05% Tween 20, 1 mM DTT, 100 uM Quinoline, 100 uM SAM, 30 min, room temperature.
Avoid freeze/thaw cycles.
GenBank
NM_006169
Mw(kda)
30 kDa
Synonyms
Nicotinamide N-Methyltransferase
Uniprot
P40261
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Tan, H.H. and Porter, A.G. Biochem. Biophys. Res. Commun. 382 (2): 462-467 (2009). 2. Fan, H. et al., World J. Gastroenterol. 15 (16): 2020-2026 (2009).