Human nicotinamide nucleotide adenylyltransferase 2, also known as NMNAT2, (GenBank Accession No. NM_015039), a.a. 2-307 (end) with N-terminal His-tag, MW = 33 kDa, expressed in an E. coli cell expression system.
Specific activity: >=23,111 pmol/min/ug
Immunogen Region
2-307
Tag
N-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3mM DTT, 375 mM imidazole
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
T50 mM Tris/HCl, pH 7.4, 5 mM MgCl2, 0.05% Tween-20, 0.1 mM NMN, 0.1mM ATP, and NMNAT2 (3.2 - 200ng) in a final volume of 50 uL. The reaction was incubated for 30 min at 37°C. The amount of ATP consumed was calculated using Kinase-Glo plus reagent (Promega).