Human nicotinamide nucleotide adenylyltransferase 1, also known as NMNAT1, (GenBank Accession No. NM_022787), a.a. 2-279 with N-terminal His-tag, MW = 32.8 kDa, expressed in an E. coli cell expression system.
Specific activity: >=4200 pmol/min/ug
Immunogen Region
2-279
Tag
N-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 256 mM imidazole and 20% glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
The 50 uL reaction mixture contained 50 mM Tris-HCl, pH 7.4, 5 mM MgCl2, 0.1 mM NMN, 0.1 mM ATP, and various amounts of NMNAT. The reaction was incubated at 37°C for 30 min, and the amount of ATP consumed was calculated using Kinase-Glo Plus reagent (Promega).