Human NEK7, also known as NIMA Related Kinase 7 (GenBank Accession No. NM_133494), full length, a.a. 1-302 (end), with N-terminal GST-tag, MW=61.5 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Host cell line: Sf9 cells
Specific activity: 47 pmol/min/ug
Immunogen Region
1-302(end)
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT and 20% glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was performed in a 25 ul reaction containing 40 mM Tris, pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA, 100 uM ATP, 0.2 mg/ml Casein and NEK7 for 50 min at 30°C. NEK7 activity was measured using ADP-Glo reagents (Promega).
Avoid freeze/thaw cycles.
GenBank
NM_133494
Mw(kda)
63 kDa
Synonyms
NEK7, NIMA-related kinase 7, Never In Mitosis-Related Kinase 7
Uniprot
Q8TDX7
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Kandli, M., et al. Genomics 68: 187-196 (2000). 2. Belham, C., et al. Curr. Biol. 15: 1155-1167 (2001).