Human NEK6 (GenBank Accession No. NM_014397), a.a. 1-313 with N-terminal His-tag, MW=40.1 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
host species: Sf9 cells
Host species: Sf9 cells
Specific activity: 70 U/mg
Unit definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol NEK6 substrate (FLAKSFGSPNRAYKK) per minute at pH 7.4 and 30°C.
Immunogen Region
1-313
Tag
N-terminal His-tag
Formulation
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na orthovanadate, 0.1 mM ATP, 4 uM NEK6 substrate, and 0.4 ug/ml NEK6.
Avoid freeze/thaw cycles.
GenBank
NM_014397
Mw(kda)
40.1 kDa
Synonyms
NEK6, NIMA-related kinase 6, Never In Mitosis-Related Kinase 6
Uniprot
Q9HC98
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Yin, M.J. et al., J. Biol. Chem. 278 (52), 52454-52460 (2003) 2. Belham, C. et al., J. Biol. Chem. 278 (37), 34897-34909 (2003)