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NEK2

SKU : BHP18200130

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BPS Bioscience

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Mfr.No.	40009
Description	Human NEK2 (GenBank Accession No. NM_002497) full length with N-terminal His-tag, MW=53 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: 100 U/mg Unit definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of myelin basic protein (MBP) substrate per minute at pH 7.4 and 30°C.
Immunogen Region	full length
Tag	N-terminal His-tag
Formulation	25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.05% Tween-20, 40% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 uM Na orthovanadate, 0.5 mM ATP, 0.3 mg/ml MBP substrate, and 0.2 ug/ml NEK2. Avoid freeze/thaw cycles.
GenBank	NM_002497
Mw(kda)	53 kDa

Synonyms	NEK2, NIMA-related kinase 2, Never In Mitosis-Related Kinase 2
Uniprot	P51955

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Fletcher, L. et al., Biochim. Biophys. Acta 1744(2):89-92 (2005). 2. Fardilha, M. et al., Ann. N.Y. Acad. Sci. 1030:468-478 (2004).Application Reference: Nek2 phosphorylates and stabilizes beta-catenin at mitotic centrosomes downstream of Plk1 (2014)

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200130	40009	10 ug	$390.50		Add to Cart

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