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Home / Proteins & Peptide

NAMPT (PBEF1), GST-tag

SKU : BHP18200859

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BPS Bioscience

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Mfr.No.	91004
Description	Human Nicotinamide phosphoribosyltransferase protein (NAMPT), also known as PBEF1 or visfatin (GenBank Accession No. NM_005746) a.a. 2-491, with N-terminal GST-tag, MW=82 kDa, expressed in an E. coli cell expression system. Specific activity: >=0.6 pmol/min/ug
Immunogen Region	2-491 (end)
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 10 mM glutathione, 0.04% Tween-20, 20% glycerol.
Species	Human
Application	Useful for the study of protein binding assays, enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes	The reaction mixture contained 50 mM Tris-HCl, pH 8.0, 12.5 mM MgCl₂, 0.1 mM nicotinamide, 0.4 mM PRPP,2 mM ATP, 30 ug/ml of alcoholdehydrogenase, 10 ug/ml of NMNAT (BPS,#71090), 1.5% alcohol, 1 mM DTT, 0.02%BSA, and various amount of NAMPT in a finalvolume of 50 ul. Reactions were initiated withthe addition of NAMPT, and the increase inNADH fluorescence (Ex 340 nm/Em 460 nm)was continuously monitored for 30 min atroom temperature. Avoid freeze/thaw cycles.
GenBank	NM_005746
Mw(kda)	82 kDa

Synonyms	Nicotinamide phosphoribosyltransferase protein, NAMPT, PBEF1, visfatin, pre-B-cell colony-enhancing factor 1, NMPT
Uniprot	P43490

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Amal B et al., Mol. Cell. Biol. 14 (2) 2. Martin PR. et al., J. Bacteriol. 183 (4)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200859	91004	50 ug	$462		Add to Cart

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