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Home / Proteins & Peptide

NAMPT (PBEF1)

SKU : BHP18200579

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Mfr.No.	71098
Description	Human Nicotinamide phosphoribosyltransferase protein (NAMPT), also known as PBEF1 or visfatin (GenBank Accession No. NM_005746), a.a. 2-491 (end), no tag, MW=56 kDa, expressed in an E. coli cell expression system. Specific activity: >= 2 pmol/min/ug
Background	Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging.
Immunogen Region	2-491(end)
Tag	none
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	The 50 ul reaction mixture contained 50 mM Tris-HCl, pH 8.0, 12.5 mM MgCl₂, 0.1 mM nicotinamide, 0.4 mM PRPP, 2 mM ATP, 30 ug/ml of alcohol dehydrogenase, 10 ug/ml of NMNAT (BPS, #71090), 1.5% alcohol, 1 mM DTT, 0.02% BSA. Reactions were incubated at room temperature for 30 minutes, and the increase in NADH fluorescence (Ex 340 nm/Em 460 nm) was measured. Avoid freeze/thaw cycles.
GenBank	NM_005746
Mw(kda)	56 kDa

Synonyms	Nicotinamide phosphoribosyltransferase protein, NAMPT, PBEF1, visfatin, Pre-B Cell-Enhancing Factor
Uniprot	P43490

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200579	71098	20 ug	$506		Add to Cart

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