Human NLR Family Pyrin Domain Containing 3 (NLRP3), also known as NALP3, Cold Autoinflammatory Syndrome 1 Protein (CIAS1), cryopyrin and PYPAF1, with K232A mutation, (Genbank Accession No. NM_004895), a.a. 2-1036(end) with N-terminal His-FLAG-tag, expressed in a baculovirus infected Sf9 cell expression system. MW = 120 kDa (calculated).
Host cell line: Sf9 cells
Immunogen Region
2-1036 (end)
Tag
N-terminal His-FLAG-tag
Formulation
40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 0.04% Tween-20, 100ug/ml FLAG peptide and 20% glycerol.
Species
Human
GenBank
NM_004895
Mw(kda)
120 kDa
Synonyms
NLR Family Pyrin Domain Containing 3, NLRP3, Cold Autoinflammatory Syndrome 1 Protein (CIAS1), cryopyrin, PYPAF1
Uniprot
Q96P20
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
Reference
1. Kummer, J.A., J. Histochem. Cytochem., 2007 May; 55(5):443-52.2. Manji, G.A., J. Biol. Chem. 2002 Mar 29; 277(13):11570-5.