Human MUSK (GenBank Accession No. NM_005592), a.a. 530-end with N-terminal His-tag, MW = 38.5 kDa, expressed in Baculovirus infected Sf9 cell expression system.
host species: Sf9 cells
Host species: Sf9 cells
Specific activity: 6.9 pmole/min/mg
Immunogen Region
530-end
Tag
N-terminal His-tag
Formulation
25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween 20, 20% glycerol and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Add 50 ul reaction mix (MUSK diluted in 1x kinase buffer supplemented with 0.2 mM ATP) to wells coated with substrate. Incubate for 1 hour. Add Europium-labeled anti p-Tyr antibody and incubate for 1 hour. Time resolved fluorescence measured at room temperature at lambdaexc=330+/-40 nm and lambdaem=620+/-10 nm.