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Home / Proteins & Peptide

MTAP, GST-tag

SKU : BHP18200391

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BPS Bioscience

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Mfr.No.	50305
Description	Human MTAP (methylthioadenosine phosphorylase), GenBank Accession No. NM_002451, a.a. 2-end, with N-terminal GST-tag, MW=57 kDa, expressed in an E. coli expression system.
Immunogen Region	2-end
Tag	N-terminal GST-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 3 mM DTT, 10% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	MTAP activity wasmeasured using a xanthine oxidase-coupledmethod. MTA cleavage by MTAP produceadenine, which is then converted to 2,8-dihydroxyadenine by xanthine oxidase,leading to an increase in absorbance at 305nm. 50 ul reaction mixtures contained MTAP,50 mM KHsub>2PO₄, pH 7.4, 200 uM MTA, 0.02units Xanthine Oxidase, and 1 mM DTT.Activity was measured at 37°C by continuousmonitoring the absorbance at 305 nm for 30min. Avoid freeze/thaw cycles.
GenBank	NM_002451
Mw(kda)	57 kDa

Synonyms	methylthioadenosine phosphorylase, MTAP
Uniprot	Q13126

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Fernandez-Irigoyen, J. et al., Biochem. J. 411 (2), 457-465 (2008) 2. Hellerbrand, C. et al., Carcinogenesis 27 (1), 64-72 (2006)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200391	50305	50 ug	$566.50		Add to Cart

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