Mouse Tryptophan 2,3-dioxygenase-2 (TDO2), GenBank Accession No.NM_019911, a.a. 2-406(end) with an NterminalHis-tag, expressed in an E. coli cellexpression system. MW = 49 kDa.
Immunogen Region
2-406(end)
Tag
N-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 300 mM imidazole
Species
Mouse
Application
Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.
Notes
Assay was performedby mixing 10 ul of varying concentrations ofmouse TDO2 with 170 ul of TDO ReactionSolution (BPS, #73005) and 20 ul of 1xTDO Assay Buffer (BPS, #73006). Thisreaction was incubated at room temperaturefor 90 min and absorbance was read at=320-325 nm.
Avoidfreeze/thaw cycles. Storing diluted enzymeis not recommended, if necessary, usecarrier protein (BSA 0.1 - 0.5%).
GenBank
NM_019911
Mw(kda)
49 kDa
Synonyms
TDO, Tryptophan 2,3-dioxygenase-2
Uniprot
P48776
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Pillotte, L., et al., Proc. Nat. Acad. Sci(USA) 2012; 109(7):2497-2502. 2. Miller, C.L., et al., Neurobiol. Disease2004; 15(3): 618-629.