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Home / Proteins & Peptide

Mouse Sphingosine kinase 1a, His-tag

SKU : BHP18200275

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BPS Bioscience

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Mfr.No.	40613
Description	Mouse Sphingosine kinase 1 (GenBank Accession No. AF415213), full length with N-terminal His-tag, MW=47.1 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 20 U/ug Unit definition: A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal His-tag
Formulation	25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Species	Mouse
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Sphingosine kinase activity was determined using D-erythro-sphingosine and ATP as substrates. Assay condition: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl₂, 1 mM DTT, 3 uM Na orthovanadate, 0.5 mM ATP, 4 uM D-erythrosphingosine, and 0.1-0.5 ug/ml sphingosine kinase 1 at 37°C for 30 min. ATP reduction is detected using Kinase-Glo Luminescent Kinase Assay Platform (Promega Corporation, Madison, WI). Avoid freeze/thaw cycles.
GenBank	AF415213
Mw(kda)	47.1 kDa

Synonyms	SphK1, sphingosine kinase 1, SPK1

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Delon, C., et al., J. Biol. Chem 279 (43) 44763-44774 (2004) 2. Taha, TA et al., FASEB J. 20(3):482-4 (2005)

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200275	40613	10 ug	$456.50		Add to Cart

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