Mouse sonic hedgehog (mShh), also knownas HHG-1. GenBank Accession No.NM_009170, a.a. 25-198 with a C-terminalHis-tag, expressed in an E. coli cellexpression system, MW = 20.5 kDa.
Formulation
10 mM Na phosphate pH 7.4, 138 mM NaCl, 2.75 mM KCl.
Species
Mouse
Application
Useful for studying protein binding and for screening small molecules and antibodies.
Endotoxin Level
<1 EU/ug
Notes
Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_009170
Mw(kda)
20.5 kDa
Synonyms
Shh; HHG-1
Uniprot
Q62226
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Tran, P.V., et al., Nat. Genet. 2008; 40:403-410. 2. Mukhopadhyay, S., et al., Cell 2012; 152(1-2): 210-223.