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Home / Proteins & Peptide

Mouse PTP1B(1-321)/PTPN, GST-tag

SKU : BHP18200029

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Mfr.No.	30012
Description	Murine recombinant PTP1B Protein tyrosine phosphatase, also known as PTPN1 (Genbank accession No. NM_011201.3), a.a. 1-321 (catalytic domain), with N-terminal GST-tag, MW=63.3 kDa, expressed in an E. coli expression system. Unit definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Background	Protein tyrosine phosphatase nonreceptor, type 1 (PTPN1) is the founding member of the protein tyrosine phosphatase (PTP) family. PTPN1 catalyze the hydrolysis of the phosphate monoesters specifically from tyrosine residues. PTPN1 is known to be involved in signaling pathways that regulate a variety of cellular processes including cell growth and oncogenic transformation. PTPN1 also has been shown to act as a negative regulator of insulin signaling. PTPN1 has also been reported to dephosphorylate epidermal growth factor receptor kinase, which implicated the role of this phosphatase in cell growth control and cell response to interferon stimulation.
Immunogen Region	1-321
Tag	N-terminal GST-tag
Formulation	25 mM Tris-HCl, 75 mM NaCl, pH 8.0, 0.05% Tween-20, 10 mM glutathione, 1 mM DTT and 50% glycerol
Species	Mouse
Application	Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Purification	Purified using glutathione-agarose beads. The purified GST fusion protein was analyzed on a Coomassie-stained SDS gel.
Notes	The activity of mouse PTP1B was determined using pNPP. Assay conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 20 mM pNPP, 5 minute incubation at 30°C, using 2 ug/ml enzyme. Avoid freeze/thaw cycles.
GenBank	NM_011201.3
Mw(kda)	63.3 kDa

Synonyms	PTP1B, PTPN1, Protein tyrosine phosphatase 1B

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	>12 months at -80°C

Reference	1. Frangioni et al., Cell, 1992, 68: 545. 2. M. Elchebly et al., Science 1999, 283: 1544.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200029	30012	50 ug was 20 ug	$357.50		Add to Cart

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