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Home / Proteins & Peptide

Mouse PKM2, His-tag

SKU : BHP18200386

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Mfr.No.	50296
Description	Murine Pyruvate kinase M2 (PKM2), also known as PKM1/M2, isoform a (GenBank Accession No. NM_011099), a.a. 2-end, with N-terminal His-tag, MW = 58 kDa, expressed in an E. coli expression system. Specific activity: >=11 pmol/min/ug.
Background	PKM1 and PKM2 are different splicing products of the PKM gene (exon 9 for PKM1 and exon 10 for PKM2). The M1 isoform (PKM1) is expressed in muscle and brain, and the M2 isoform is expressed during embryogenesis, in adipose tissue and pancreatic islets, and is the predominant form found in cancer cells.
Immunogen Region	2-end
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Species	Mouse
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	A 50 ul PKM2 reaction is conducted in abuffer containing 50 mM Tris (pH 7.4),100 mM MgCl₂, 250 mM KCl, 40 mM ADP,100 mM phosphoenolpyruvate (PEP) atroom temperature for 15 min. ATPproduction is detected using Kinase-GloLuminescent Kinase Assay Platform. Avoid freeze/thaw cycles.
GenBank	NM_011099
Mw(kda)	58 kDa

Synonyms	pyruvate kinase muscle, PKM2, cytosolic thyroid hormone-binding protein,CTHBP, thyroid hormone-binding protein 1, THBP1, opa-interacting protein 3,OIP3
Uniprot	P52480

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Spoden G.A. et al., Exp. Cell Res. 315 (16), 2765-2774 (2009). 2. Christofk H.R. et al., Nature 452 (7184), 230-233 (2008).

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200386	50296	20 ug	$555.50		Add to Cart

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