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Home / Proteins & Peptide

Mouse PDE5A1, GST-tag

SKU : BHP18200515

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BPS Bioscience

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Mfr.No.	60051
Description	Mouse PDE5A (GenBank Accession No. NM_153422), full length, with N-terminal GST-tag, MW=124 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 4630 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 18mM glutathione, 10% glycerol.
Species	Mouse
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 mM Tris-HCl,pH 7.4, 10 mM MgCl₂, 1 mM MnCl₂, 0.1 mg/mlBSA, 200 uM cGMP, 2.5 kU 5'-nucleotidase, andserial dilutions of mPDE5A at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -GMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_153422
Mw(kda)	124 kDa

Synonyms	PDE5A, phosphodiesterase 5A
Uniprot	Q8CG03

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Das, A. et al., J. Biol. Chem. 280 (13), 12944-12955 (2005). 2. Champion, H.C.et al., Proc. Natl. Acad. Sci. U.S.A. 102 (5), 1661-1666 (2005).

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200515	60051	5 ug	$500.50		Add to Cart

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