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Home / Proteins & Peptide

Mouse PDE1C, GST-tag

SKU : BHP18200493

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BPS Bioscience

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Mfr.No.	60012
Description	Mouse PDE1C (GenBank Accession No. NM_001025568), full length with N-terminal GST-tag, MW= 100 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 16,650 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 3 mM DTT, 10% glycerol
Species	Mouse
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assayed using BPS's Mouse PDE1C Assay Kit, Cat. # 61312. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA,0.05% Tween-20, 200 uM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE1C at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_001025568
Mw(kda)	100 kDa

Synonyms	PDE1C, phosphodiesterase 1C
Uniprot	Q64338

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Gong S. et al., Nature 425 (6961), 917-925 (2003). 2. Spence S. et al., Biochem. J. 321 (PT 1), 157- 163 (1997).

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200493	60012	10 ug	$500.50		Add to Cart

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