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Home / Proteins & Peptide

Mouse PDE10A, GST-tag

SKU : BHP18200536

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BPS Bioscience

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Mfr.No.	60101
Description	Mouse PDE10A GenBank Accession No. NM_011866), full length with N-terminal GST-tag, MW=117 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 Specific activity: >= 1,029 pmol/min/ug Unit definition: One unit is defined as the amount of enzymethat will convert 1 pmole of 3' , 5' -cAMP to5' -AMP per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Mouse
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂,0.1 mg/ml BSA, 0.05% Tween-20, 200 uMcAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE at 37°Cfor 20 min. Quantified by 5'-nucleotidasecleaving the 5 -AMP product and releasing the phosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_011866
Mw(kda)	117 kDa

Synonyms	PDE10, phosphodiesterase 10A
Uniprot	Q8CA95

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Siuciak, J.A. et al., Neuropharmacology 51 (2), 374-385 (2006). 2. Hu,H. et al., Eur. J. Neurosci. 20 (12), 3351-3363 (2004).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200536	60101	10 ug	$500.50		Add to Cart

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