Murine recombinant IL2-inducible T-cell kinase (ITK), also known as Lyk and TSK (GenBank Accession #NM_010583), a.a. 352-620(end) with N-terminal GST-tag, MW= 57 kDa, expressed in Sf9 cells via a baculovirus expression system.
Host cell line: Sf9 cells
Specific activity: 2.4 pmol/min/ug
Immunogen Region
352-620(end)
Tag
N-terminal GST-tag
Formulation
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 3 mM DTT, and 10% glycerol.
Species
Mouse
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Z-Lyte (Invitrogen) screening protocol and condition were used. Enzyme reaction was conducted at 30°C for 1 hours with 2 uM Tyr 01 Peptide in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. After the 1 hour incubation, diluted Development Reagent B was added.