Mouse IDO1, also known as Indoleamine 2,3-dioxygenase 1, GenBank Accession No. NM_008324, a.a. 2-407(end) with an Nterminal His-tag, expressed in the presence of hemin in an E. coli cell expression system. MW = 46 kDa.
Immunogen Region
2-407
Tag
Nterminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species
Mouse
Application
Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.
Notes
Assay was performed in20 mM ascorbate, 10 mM methylene blue,10 ug/ml catalase, 100 uM NaPi, pH 6.5containing varying concentrations oftryptophan. 300 ng of IDO1 was added toinitiate reaction and aliquots were collectedat various time points for Kyn analysis.
Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank
NM_008324
Mw(kda)
46 kDa
Synonyms
Indoleamine 2,3-dioxygenase 1, IDO, IDO1
Uniprot
P28776
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C (for all proteins)
Reference
1. Lob, S. et al., Cancer Immunol. Immunother. 2009; 58(1): 153-157. 2. Liu, X. et al., Blood. 2010; 115(17): 3520-3530. 3. Flick, H.E., et al., Int. Nat. J. Tryptophan Res. 2013; 6: 35-45.