Mouse EGFR (GenBank Accession No. NM_207655/NP_997538), a.a. 670-1210 with a C-terminal FLAG-tag, MW = 62 kDa, expressed in SF9 cells via a baculovirus expression system.
Host cell line: Sf9 cells
Specific activity: 49 pmole/min/mg.
Immunogen Region
670-1210
Tag
C-terminal FLAG-tag
Formulation
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol
Species
Mouse
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was performed in a Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 uM ATP. The reaction to place at 30°C for 40 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Avoid freeze/thaw cycles.
GenBank
NM_207655
Mw(kda)
62 kDa
Synonyms
EGFR, Epidermal Growth Factor Receptor, ERBB, ErbB-1, HER1, MENA
Uniprot
Q01279
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Mackenzie, J. et al., Mol Immunol. 2011 Jul,48(12-13):1578-85 2. Sadri, K. et al., Nucl Med Commun. 2011 Jul,32(7):563-9.