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Home / Proteins & Peptide

MMP9 (Q279R), His-tag

SKU : BHP18200806

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Mfr.No.	80215
Description	Human matrix metallopeptidase-9 (MMP9), also known as gelatinase B, MANDP2, CLG4B, Macrophage gelatinase, GELB and EC 3.4.24, with Gln to Arg mutation on a.a. 279, GenBank Accession No. NM_004994), a.a. 20-707 (end) with C-terminal His-tag, MW=77.8 kDa, expressed in an HEK293 cell expression system. Specific activity: >= 2500 pmole/min/ug
Immunogen Region	20-707 (end)
Tag	C-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol
Mutation	Q279R
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Activation: This is a proform enzyme andrequires activation prior to testing activity. Dilutethe enzyme to 100 ng/ml in a solution containing50 mM HEPES, pH 7.4, 10 mM CaCl2, 0.05%Brij-35, and 1 mM APMA (amino-phenylmercuric acetate). Incubate at 37°C for 2 hours. Assay conditions: Reaction mixture with 10 uM390 MMP substrate 1 (Anaspec). Incubate for30 minutes at room temperature. Fluorescenceintensity is measured at exc328/em393. Avoid freeze/thaw cycles.
GenBank	NM_004994
Mw(kda)	77.8 kDa

Synonyms	matrix metallopeptidase-9, MMP9, gelatinase B, MANDP2, CLG4B, Macrophage gelatinase, GELB, EC 3.4.24, MMP
Uniprot	P14780

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Wenham, R.M., et al., J. Soc. Gynecol.Investig. 2003 Sep;10(6):381-7. 2. Li, X., et al., J. Cancer Res. Clin. Oncol.2013 Sep;139(9):1433-47.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200806	80215	20 ug	$467.50		Add to Cart

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