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Home / Proteins & Peptide

MMP2, His-tag (HEK293-derived)

SKU : BHP18200804

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BPS Bioscience

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Mfr.No.	80213
Description	Human matrix metallo-peptidase-2 (MMP2), also known as gelatinase A (GenBank Accession No. NM_004530), a.a. 30 - 660 (end), with C-terminal His-tag, MW=72 kDa, expressed in an HEK293 cell expression system. Specific activity: >= 511 pmole/min/ug
Immunogen Region	30 - 660 (end)
Tag	C-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Activation: This is a proform enzyme andrequires activation prior to testing activity.Dilute the enzyme to 100 ng/ml in a solutioncontaining 50 mM HEPES, pH 7.4, 10 mMCaCl2, 0.05% Brij-35, and 1 mM APMA(amino-phenyl mercuric acetate). Incubate at37°C for 2 hours. Assay conditions: Reaction mixture with 10 uM390 MMP substrate 1 (Anaspec). Incubate for30 minutes at room temperature. Fluorescenceintensity is measured at exc328/em393. Avoid freeze/thaw cycles.
GenBank	NM_004530
Mw(kda)	72 kDa

Synonyms	matrix metallo-peptidase-2, MMP2, gelatinase A, MMP
Uniprot	P08253

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Van Dijk, M., et al.,Front Genet.2012; 3:22.2. Klotzsche-von Ameln, A., et al.,Cancer Biol Ther. 2012 Feb 15;13(4): 216 - 223.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200804	80213	20 ug	$467.50		Add to Cart

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