Human matrix metallopeptidase-1 (MMP1), also known as CLG, Interstitial Collagenase, Fibroblast Collagenase, and EC 3.4.24.7 (GenBank Accession No. NM_002421), a.a. 20-469 (end), with C-terminal His-tag, MW=53 kDa, expressed in an HEK293 cell expression system.
Specific activity: >= 105 pmole/min/ug
Immunogen Region
20-469 (end)
Tag
C-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Activation: This is a proform enzyme andrequires activation prior to testing activity. Dilutethe enzyme to 100 ng/ml in a solution containing50 mM HEPES, pH 7.4, 10 mM CaCl2, 0.05%Brij-35, and 1 mM APMA (amino-phenylmercuric acetate). Incubate at 37°C for 2 hours. Assay conditions: Reaction mixture with 10 uM390 MMP substrate 1 (Anaspec). Incubate for30 minutes at room temperature. Fluorescenceintensity is measured at exc328/em393.