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Home / Proteins & Peptide

MLL3/WDR5/Ash2L/RbBP5/DPY30 Complex

SKU : BHP18201821

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BPS Bioscience

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Mfr.No.	100323
Description	MLL3/WARD complex of human MLL3 (GenBank Accession No. NM_170606) a.a. 4689-4911(end) with N-terminal His-tag and MW= 27 kDa, human WDR5 (GenBank Accession No. NM_017588), a.a. 2-334(end ) with N-terminal His-tag and MW= 37 kDa, human Ash2L (GenBank Accession No. NM_001105214), a.a. 2-534(end) with N-terminal His-tag and MW= 61 kDa, human RbBP5 (GenBank Accession No. NM_005057), a.a. 2-538(end) with N-terminal His-tag and MW= 60 kDa, and human DPY30 (GenBank Accession No. NM_032574) a.a. 2-99(end) with N-terminal His-tag and MW=12 kDa individually expressed in an E. coli expression system.
Immunogen Region	MLL3 4689-end other components 2-end
Tag	N-terminal His-tag (all)
Formulation	40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 200 mM imidazole, 1 uM ZnCl₂, 1mM TCEP.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	H3, 1-21-Biotin was coated onto a neutravadin plate at 0.4 ug/ml. Reaction mixture contained 1X HMT buffer 1 (50 mM Tris buffer pH 8.8, 5 mM MgCl2, 4 mM DTT, 0.05% Tween-20), various amounts of S-adenosylmethionine (40 uM for SA determination, 20 uM for inhibition), and/or various amounts of MLL3 Complex-incubated for 1-hour at room temperature. Followed by 1-hour incubation with primary antibody against 3MeK4 Histone 3 (cell signaling #9751) and 30-minute incubation with alpha-rabbit secondary antibody (abcam). Luminescence was developed with ELISA ECL Substrates A and B (BPS #79670).
GenBank	NM_170606 (MLL3), NM_017588 (WDR5), NM_001105214 (Ash2L), NM_005057 (RbBP5), NM_032574 (DPY30)
Mw(kda)	183 kDa (complex)

Synonyms	KMT2C, HALR, MLL3, lysine methyltransferase 2C, KLEFS2
Uniprot	MLL3: Q8NEZ4; WDR5: P61964; Ash2L: Q9UBL3; RbBP5: Q15291; DPY30: Q9C005

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Patel, A., et al., J. Biol. Chem. 2009; 284(36): 24242-24256. 2. Mohan, M., et al., Nat. Rev. Cancer 2010; 10(10): 721-728.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201821	100323	50 ug	$737		Add to Cart

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