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Home / Proteins & Peptide

MGLL, His-tag, GST-tag

SKU : BHP18200578

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Mfr.No.	71096
Description	Human monoglyceride lipase (MGLL), GenBank Accession No. NM_007283, a.a. 2-313(end), with N-terminal His-tag and N-terminal GST-tag, MW=62 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Background	Monoacylglycerol lipase (MAGL) catalyzes hydrolysis of intracellular triglyceride stores, forming fatty acids and glycerol. It is a key enzyme in the metabolism of 2-arachidonoylglycerol, hence is involved in a similar pathway to DAGL. MAGL is localized in the enteric nervous system where it functions to regulate levels of endocannabinoids, and as a result, regulates intestinal motility. It is also found in the gut epithelium where it may have a role in digestion.
Immunogen Region	2-313(end)
Tag	N-terminal His-tag, N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 228 mM imidazole, 0.04% Tween-20, 3 mM DTT, and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reaction was performed in 50 ul reaction mix containing 50 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM 4-Methylumbelliferyl butyrate, and various amounts of MGLL. Reaction was incubated at 37°C for 15 minutes and fluorescent signal was measured at ex 360 nm, em 445 nm. Avoid freeze/thaw cycles.
GenBank	NM_007283
Mw(kda)	62 kDa

Synonyms	monoglyceride lipase, MGLL, HUK5, MAGL, Monoacylglycerol Lipase
Uniprot	Q99685

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Sun, H., et al., Oncogene 2013;32(2): 234-241. 2. Labar, G., et al., Chembiochem. 2010;11(2):218-227.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200578	71096	50 ug	$407		Add to Cart

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