Human MEKK2, also known as MAP3K2 and MEKK2B, GenBank Accession No. NM_006609, full length, N-terminal GST-tag, MW=96.5 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Host cell line: Sf9 cells
Specific activity: 18 pmol/min/ug
Immunogen Region
full length
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl , 20% glycerol and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA and 1 mM DTT) containing 20 uM ATP and 0.2 mg/ml poly-(Glu-Tyr) for 30 minutes at 30°C. Enzyme activity is measured using Kinase-Glo Plus reagents (Promega Corporation, Madison, WI).
Avoid freeze/thaw cycles.
GenBank
NM_006609
Mw(kda)
96.5 kDa
Synonyms
MEKK2B, MAP3K3, MAPKKK3, Mitogen-Activated Protein Kinase Kinase Kinase 3
Uniprot
Q9Y2U5
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Blank, J. L. et al. J. Biol. Chem. 271:5361-5368, 1996. 2. Hammaker, D. R. et al. J. Immun. 172:1612-1618, 2004.