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Home / Proteins & Peptide

MEG-2 (PTPN9), GST-tag

SKU : BHP18200032

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Mfr.No.	30020
Description	Protein tyrosine phosphatase (GenBank accession number M83738), a.a. 285-593 (catalytic domain) with N-terminal GST-tag, MW=60.3 kDa, expressed in an E. coli expression system. Specific activity: 1070 pmole/min/ug
Background	This PTP contains an N-terminal domain that shares a significant similarity with yeast SEC14, which is a protein that has phosphatidylinositol transfer activity and is required for protein secretion through the Golgi complex in yeast. This PTP was found to be activated by polyphosphoinositide, and is thought to be involved in signaling events regulating phagocytosis.
Immunogen Region	285-593
Tag	N-terminal GST-tag
Formulation	25 mM Tris-HCl, pH 8.0, 67 mM NaCl, 1.4 mM KCl, 50% glycerol, 10 mM glutathione, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Notes	20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and 2 ng/ul MEG-2. Assay was done at RT for 15 min, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on epsilon_pNPP= 18,000 M^-1cm^-1. Avoid freeze/thaw cycles.
GenBank	M83738
Mw(kda)	60.3 kDa

Synonyms	MEG2, MEG-2, PTPN9
Uniprot	P43378

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	>12 months at -80°C

Reference	1. Q. Ying, et al., J. Cell Biochem. 2002, 86: 79. 2. H. Huynh, et al. J. Immunol. 2003, 171: 6661.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200032	30020	50 ug was 20 ug	$357.50		Add to Cart

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