Recombinant human MDM2 (Mouse double minute 2 homolog), full length encompassing amino acids 2-498 (end). In this construct the N-terminal end of MDM2 is fused with GST. A short peptide linker placed between GST and the protein includes a PreScission Protease Cleavage Site to facilitate removal of the GST portion. The recombinant protein was affinity purified. Constructs: MDM2 (GST-Pre-2-498(end))
Immunogen Region
2-498(end)
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was performed according to MDM2 TR-FRET Assay Kit (Biohippo #79773) with MDM2 titrated from 157.5 ng - 0 ng.