Recombinant rabbit MCL1 (induced myeloid leukemia cell differentiation protein Mcl-1), encompassing amino acids 2-329. This construct contains an N-terminal His-tag (6xHis). The recombinant protein was affinity purified. Constructs: MCL1 (His-2-329) (Rabbit)
host species: E. coli
Host species: E. coli
Immunogen Region
2-329
Tag
N-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT
Species
Rabbit
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes
Assay run according to MCL-1 TR-FRET Assay Kit (Biohippo Kit #79506). Enzyme mixture incubated for 1 hour at room temperature and TR-FRET measured at ex 340 nm / em 620 nm, 665 nm.