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Home / Proteins & Peptide

MAT2A, His-Tag

SKU : BHP18201674

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Mfr.No.	71401-1
Description	Human MAT2A, also known as methionine adenosyltransferase II alpha, MAT2, and adoMet synthase 2. GenBank Accession No. NM_005911, a.a. 2-359 (end) with an N-terminal His-tag, expressed in an E. coli cell expression system. MW = 45 kDa.
Immunogen Region	2-359 (end)
Tag	N-terminal His-Tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 167 mM imidazole
Species	Human
Application	Useful for studying protein binding and screening small molecular inhibitors
Notes	The reactions were conducted at 30°C for 30 minutes in a 25 ul mixture containing 40 mM Tris-HCl, pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA, various amount of MAT2A enzyme, 0.1 mM L-Methionine, and 10 uM ATP. After enzymatic reactions, 25 ul of Kinase-Glo MAX reagent was added to each well and luminescence was measured using a BioTek Synergy 2 microplate reader. Avoid freeze/thaw cycles.
GenBank	NM_005911
Mw(kda)	45 kDa

Synonyms	MATA2; MATII; SAMS2; S-adenosylmethionine synthase isoform type-2; methionine adenosyltransferase II, alpha; MAT 2; MAT-II; adoMet synthetase 2; methionine adenosyltransferase 2; adoMet synthase 2; testicular tissue protein Li 121
Uniprot	P31153

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.

Reference	1. Latasa, M., et al. The FASEB Journal. 2001; 15(7): 1248-1250.2. Liu, Q., et al., Molecular Cancer Therapeutics. 2011; 10(6):1113-23.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201674	71401-1	100 ug	$434.50		Add to Cart

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