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Home / Proteins & Peptide

MAPK14 (p38alpha), His-tag, active

SKU : BHP18200202

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BPS Bioscience

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Mfr.No.	40243
Description	Mitogen-activated proteinkinase 14 (MAPK14) GenBankAccession No. NM_139012, a.a. 2-360(end), with N-terminal His-tag, MW 42.3kDa, expressed in a baculovirus-infectedSf9 cell expression system. This proteinis activated by in vitro phosphorylationand SEC. Host cell line: Sf9 cells Specific activity: 117 pmol/min/ug
Immunogen Region	2-360
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% glycerol
Species	Human
Application	Useful for studying enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes	Assay was done in Kinase buffer containing 0.2 mM DTT using P38 peptide substrate (0.1 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega) Storing diluted enzymeis not recommended, if necessary, usecarrier protein (BSA 0.1 - 0.5%). Avoid freeze/thaw cycles.
GenBank	NM_139012
Mw(kda)	42.3 kDa

Synonyms	Mitogen-activated protein kinase 14, p38-alpha, p38alpha
Uniprot	Q16539

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C. Avoidfreeze-thaw cycles.

Reference	1. Hatherley, D., et al., Mol. Cell, 2008,31:266-77 2. Baba, N., et al., J. Exp. Med., 2013,210(6):1251-63

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200202	40243	20 ug	$423.50		Add to Cart

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