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Home / Proteins & Peptide

MAPK10 (JNK3), GST-tag

SKU : BHP18200166

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Mfr.No.	40092
Description	Human MAPK10 (mitogen-activated protein kinase 10), also known as PRKM10, JNK3 (c-Jun N-terminal kinase-3), or SAPKbeta (stress activated protein kinase-beta), GenBank Accession No. NM_002753, a.a. 2-422 (end), with N-terminal GST-tag, MW=75 kDa, expressed in Sf9 cells via a baculovirus expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: 22 pmol/min/ug
Immunogen Region	2-422 (end)
Tag	N-terminal GST-tag
Formulation	25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Enzyme reaction was performed in 50 ul kinase reaction buffer (40 mM Tris pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.5 mM DTT) containing 50 uM ATP, 0.2 mg/ml ATF2 (BPS #40520) and 2-16 ng/ul MAPK10 for 30 minutes at 30°C. The amount of ATP transferred was assayed using the Biohippo ATP quantification reagents. Avoid freeze/thaw cycles.
GenBank	NM_002753
Mw(kda)	75 kDa

Synonyms	MAPK10, mitogen-activated protein kinase 10, JNK3, c-Jun N-terminal kinase 3, SAPKbeta, stress activated protein kinase beta, PRKM10
Uniprot	P53779

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Lisnock J, et al. (2000). Biochemistry 39(11): 3141-8. 2. Hu MC, et al. (1997). Oncogene 15(19): 2277-87. 3. Ying J, et al. (2006). Leukemia 20(6): 1173-1175.

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200166	40092	10 ug	$390.50		Add to Cart

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