Human Lysyl oxidase homolog 4 (LOXL4) also known as, Lysyl oxidase-related protein C (LOXC). GenBank Accession No. NM_032211, a.a. 570-756(end) with a C-terminal His-tag expressed in an E. coli expression system.
Formulation
9 mM Na2HPO4/KH2 PO4 pH 7.4, 124 mM NaCl, 2.4 mM KCl and 10% glycerol
Species
Human
Application
Useful as an immunogen and for protein binding studies.
Notes
Enzymatic activity was confirmed by an AmplexRed/Peroxidase coupling method using 1,5-diaminopentane as a substrate. Purified LOXL4 (0.25-1 ng/ul) was incubated with 3 mM 1,5 diaminopentane, 0.4 mM AmplexRed and 0.2 unit/ul peroxidase in 100 ul of the assay buffer (1.2 M Urea, 50 mM borate pH 8.8) for 15 min at 30°C. Fluorescence intensity is measured every 7 min at 530ex/590em.
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank
NM_032211
Mw(kda)
23 kDa
Synonyms
Lysyl oxidase-related protein C, LOXC
Uniprot
Q96JB6
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Gorogh, T., et al., Int. J. Oncol. 2008; 33(5): 1091-1098. 2. Kim, D.J., et al., Biochem. Biophys. Res. Commun. 2008; 4: 521-527.