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Home / Proteins & Peptide

LSD2 (KDM1B), GST-Tev-Tag

SKU : BHP18200348

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BPS Bioscience

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Mfr.No.	50124
Description	Human LSD2 protein, also known as KDM1B, GenBank Accession No. NM_153042, a.a. 2-822 with N-terminal GST-Tev-Tag. MW = 121 kDa, expressed in Sf9 cells via a baculovirus expression system. Host cell line: Sf9 cells
Immunogen Region	2-822
Tag	N-terminal GST-Tev Tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, and 1.6 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 uL of reaction mixture contains 50 mM Tris, pH 8.8, 50 mM NaCl, 0.01% Tween-20, 0.5 uM biotinylated peptide substrate, and various amount of LSD2. The reactions were incubated at room temperature for one hour with slow shaking. After the reaction, 10 uL of primary antibody (1:800 dilution) and anti-mouse acceptor beads (1:500 dilution) was added into the wells and the reactions were incubated for 30 min. Then 10 uL of Streptavidin-conjugated donor beads (1:125 dilution) was added into the wells. The reactions were incubated for additional 30 min at room temperature followed by Alpha screening detection. Avoid freeze/thaw cycles.
GenBank	NM_153042
Mw(kda)	121 kDa

Synonyms	KDM1B
Uniprot	Q8NB78

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).

Reference	1. Binda, C., et al., Journal of American Chemical Society. 2010; 132(19): 6827-6833.2. Welte, M., et al., Cell Press. 2005; 15(14): 1266-1275.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200348	50124	20 ug	$522.50		Add to Cart

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