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Home / Proteins & Peptide

LSD1 (KDM1A), GST-tag, Variant 2

SKU : BHP18200333

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BPS Bioscience

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Mfr.No.	50100
Description	Human LSD1 (also known as KDM1A (lysine (K)-specific demethylase 1A) or AOF2, amine oxidase (flavin containing) domain 2)) GenBank Accession No. NM_015013, a.a. 158-end with N-terminal GST-tag, MW=103 kDa, expressed in an E. coli expression system. Specific activity: >=0.50 pmole/min/ug
Immunogen Region	158-end
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 ul reaction mix containing assay buffer with 50 mM Tris HCl pH 8.8, 50 mM NaCl, 1mM DTT, 0.01% Tween-20, histone H3 peptide substrate, and LSD1 (6.7 - 40 ng) add to the wells. Incubate for 1 hr. Add antibody against unmethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection. Important: If you intend to assess LSD1 activity in an assay that analyzes hydrogen peroxide, such as BPS Catalog #50106 and #50107, please include a request for Peroxidase Rxn Grade LSD1 with your order. Avoid freeze/thaw cycles.
GenBank	NM_015013
Mw(kda)	104 kDa

Synonyms	KDM1A, AOF2, lysine (K)-specific demethylase 1A, amine oxidase (flavin containing) domain 2, LSD
Uniprot	O60341

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Lan,F. et al., Nature 448, 7154, 718-722, 2007. 2. Forneris,F. et al., J. Biol. Chem. 282, 28, 20070-20074, 2007.Application Reference(s): 1.A scintillation proximity assay for histone demethylases (2014) 2.Crystal structure of the histone lysine specific demethylase LSD1 complexed with tetrahydrofolate (2014)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200333	50100	50 ug	$522.50		Add to Cart

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