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Home / Proteins & Peptide

LKB1/MO25/STRAD Complex, STK11, His-tag, MO25, GST-tag, STRAD

SKU : BHP18200152

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Mfr.No.	40069
Description	Complex of human LKB1, also known as STK11 (GenBank Accession No. NM_000455, a.a. 2-433, with N-terminal His-tag), MO25 (GenBank Accession No. NM_016289, a.a. 2-341, with N-terminal GST-tag), and STRAD (GenBank Accession No. NM_001003787, a.a. 2-431, with N-terminal GST-tag). Calculated MW=49 kDa* (LKB1), 66 kDa (MO25), and 74 kDa (STRAD), expressed in Sf9 cells using a Baculovirus expression system. *LKB1 runs anomalously on SDS-PAGE. host species: Sf9 cells Host species: Sf9 cells Specific activity: 24 pmol/min/ug
Immunogen Region	LKB1 2-433, MO25 2-341, STRAD 2-431
Tag	STK11, N-terminal His-tag, MO25, N-terminal GST-tag, STRAD, N-terminal GST-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 3 mM DTT, 10% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	100 uM ATP and 0.2 mg/ml LKBtide peptide was used as a substrate. Reaction was incubated at 30°C for 30 min. Reaction progress was monitored using ADP-Glo reagents (Promega). Avoid freeze/thaw cycles.
GenBank	STK11 (NM_000455), MO25 (NM_016289), STRAD (NM_001003787)
Mw(kda)	189 kDa (complex)

Synonyms	LKB1, STK11, PJS, Liver Kinase B1, STRAD, STE20-related kinase adaptor, LYK5, PMSE, MO25, Calcium Binding Protein 39
Uniprot	LKB1: Q15831; MO25: Q9Y376; STRAD: Q7RTN6

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Yoo, L.I., et al. (2002). Nat. Rev. Cancer 2(7): 529-535. 2. Katajisto P, et al. (2007). Biochim. Biophys. Acta 1775 (1): 63-75.

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200152	40069	20 ug	$407		Add to Cart

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