Human LIGHT protein, also known as TNFSF14, GenBank Accession No. NM_003807, a.a. 74-240 with N-terminal His-tag. MW = 21 kDa, expressed in Sf9 cells via a baculovirus expression system. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Immunogen Region
74-240
Tag
N-terminal FLAG-Tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, and 140 mM Imidazole
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Avoid freeze/thaw cycles.
GenBank
NM_003807
Mw(kda)
21 kDa
Uniprot
O43557
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Reference
1. Garrido, V., et al., British Journal of Maematology. 2012; 158(6): 788-797.2. Ogawa, T., et al., Journal of Urology. 2010; 183(3): 1206-1212.