JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Proteins & Peptide

LIGHT His-Avi-Tag, Biotin-labeled

SKU : BHP18201092

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

BPS Bioscience

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	100032-1
Description	Human LIGHT, also known as TNFSF14, HVEM-L, and LT-y, GenBank Accession No. NM_003807, a.a. 72-240(end) with an N-terminal His-tag and C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag technology. Biotinylation is confirmed to be>=90%. MW=23 kDa. This protein runs at a higher molecular weight due to glycosylation.
Tag	N-terminal His-tag and C-terminal Avi-tag
Formulation	8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, 20% glycerol.
Species	Human
Application	Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.
Notes	Reaction done according to LTBR:LIGHT(B) assay kit (BPS Biosience catalog # 79808) protocol. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock PBS + 0.05% Tween-20. Coat protein (LTBR) was added overnight at 4 deg 100 ng/well. Various amounts of biotinylated ligand (LIGHT(B)) (0-240 ng) added for 1h at RT. Strep-HRP added for 1h at RT. Luminescence developed with ELISA ECL substrate (Biohippo catalog #79670). Avoid freeze/thaw cycles.
GenBank	NM_003807
Mw(kda)	26 kDa

Synonyms	TNFSF14, HVEM-L, LT-y,
Uniprot	O43557

Shipping Temperature	-80°C (dry ice)
Storage	At least 6 months at -80°C.

Reference	1. Edwards, J.R. et al., Arthritis & Rheumatology. 2006; 54(5): 1451-1462. 2. Ogawa, T. et al., The J. or Urology. 2010; 183(3): 1206-1212.

Product Manual	Datasheet

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHP18201092	100032-1	25 ug	$324.50		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.