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Home / Proteins & Peptide

LIF, Mouse

SKU : BHP18500251

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Mfr.No.	IX03077, IX03077-1, IX03077-10, IX03077-50
Description	Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine belonging to the long four-helix bundle cytokine superfamily. LIF shares tertiary structure with several other cytokines, including Interleukin-6 (IL-6), Oncostatin M, ciliary neurotropic factor, and cardiotrophin-1, and their functions in vivo are also redundant to some extent. LIF can bind to the common receptor of IL-6 subfamily, gp130, and then recruit its own receptor LIF Receptor to form a ternary complex. The basal expression of LIF in vivo is low; and its expression is induced by pro-inflammatory factors, including lipopolysaccharide, IL-1, and IL-17, and inhibited by anti-inflammatory agents, including IL-4 and IL-13. The functions of LIF include proliferation of primordial germ cells, regulation in blastocyst implantation and early pregnancy, and maintenance of pluripotent embryonic stem cells.Recombinant mouse Leukemia Inhibitory Factor (rmLIF) produced in E. coli is a single non-glycosylated polypeptide chain containing 180 amino acids. A fully biologically active molecule, rmLIF has a molecular mass of 19.9 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.
Reconstitution	Reconstituted in ddH2O at 100 ug/mL.
Formulation	Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH8.0.
Molecular Weight	19.9 kDa, observed by reducing SDS-PAGE.
Source	E. coli
Host	Mouse
Accession Number	P09056
Endotoxin Level	0.2 EU/ug, determined by LAL method.
Notes	For research use only
Bioactivity	ED50 0.01 ng/mL, measured by a cell differentiation assay using M1 cells, corresponding to a specific activity of 1.0x 108 units/mg.

Synonyms	CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA
Sequence	SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANALFISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKLVELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATIDVMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKKLGCQLLGTYK QVISVVVQAF SPLPITPVNA TCAIRHPCHGNLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLCAPNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRDQKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVGHVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF

Purity	95% by SDS-PAGE and HPLC analyses.
Storage	Lyophilized recombinant mouse Leukemia Inhibitory Factor (rmLIF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmLIF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500251-1	IX03077-1	1mg	$2746		Add to Cart
BHP18500251-2	IX03077-10	10ug	$107		Add to Cart
BHP18500251-3	IX03077-50	50ug	$406		Add to Cart

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