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LDLR, FLAG-tag

SKU : BHP18200637

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Mfr.No.	71205
Description	Human low density lipoprotein receptor (LDLR), also known as FH, FHC, and LDLCQ2, GenBank Accession No. NM_000527, a.a. 22-788(end), with C-terminal FLAG-tag, MW=87.4 kDa* (calculated), expressed in an HEK293 cell expression system.
Background	The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. In case of HIV-1 infection, it functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of LDLR, inducing LDLR degradation.
Immunogen Region	22-788(end)
Tag	C-terminal FLAG-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species	Human
Application	Useful for studying protein binding and screening small molecules for drug discovery.
Notes	50 uL reaction mix (50 mM HEPES pH 7.4, 100 mM NaCl, 200 uM CaCl₂, 0.05% Tween-20, 0.1% BSA, and up to 1500 ng PCSK9) is added to the wells coated with LDLR Ectodomain. Incubate for 2hr. Add antibody against polyhistidine and then incubate 1 hr. Add secondary HRP labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence. * LDLR is heavily glycosylated, resulting in higher molecular weight. The two bands shown correspond to differing states of glycosylation. Avoid freeze/thaw cycles.
GenBank	NM_000527
Mw(kda)	87.4 kDa

Synonyms	FH, FHC, LDLR, LDLCQ2, low density lipoprotein receptor
Uniprot	P01130

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Holla, L., et al., BMC Cell Biol. 2007 Mar 1,8:9. 2. Qian, YW., et al., J Lipid Res. 2007 Jul,48(7):1488-98. 3. Fasano, T., et al., Athersclerosis. 2009 Mar,203(1):166-71.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200637	71205	50 ug	$511.50		Add to Cart

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