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LDHB, His-tag

SKU : BHP18200565

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Mfr.No.	71078
Description	Human lactate dehydrogenase B (LDHB) a.a. 2 - 334 (end) (GenBank Accession No. NM_002300) with N-terminal His-tag, MW = 37.5 kDa, expressed in an E. coli expression system. Specific activity: >=350 nmole/min/ug.
Immunogen Region	2 - 334 (end)
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 240 mM imidazole, 0.04% Tween-20, and 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reaction was conducted in 100 mM Tris, pH 7.4, 2 mM sodium pyruvate, 0.2 mM beta-NADH, 0.02% BSA, and LDHB in a volume of 200 uL. Initiate reactions with LDHB. Monitor the decrease in NADH absorbance at 340 nm at 37°C for 5 min. using Tecan Infinite 1000. Avoid freeze/thaw cycles.
GenBank	NM_002300
Mw(kda)	37.5 kDa

Synonyms	lactate dehydrogenase B, LDHB
Uniprot	P07195

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Armstrong, A.J., et al. J Clin Oncol. 2012 Sep 30;30(27):3402-7. 2. Sudo, K., et al., Hum Genet. 1992 May;89(2):158-62.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200565	71078	100 ug	$407		Add to Cart

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