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Home / Proteins & Peptide

LDHA, His-tag

SKU : BHP18200567

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BPS Bioscience

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Mfr.No.	71081
Immunogen Region	2-332(end)
Tag	N-terminal His-tag
Description	Human lactate dehydrogenase A (LDHA), GenBank Accession No. NM_005566), full length (2-332(end)) with N-terminal His-tag, MW= 37.5kDa, expressed in an E. coli expression system. Specific activity: >=700 nmole/min/ug.
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 160 mMimidazole, 0.04% Tween-20, and 20%glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reaction was conducted in 100 mM Tris, pH 7.4, 2 mM sodium pyruvate, 0.2 mM beta-NADH, 0.02% BSA, and LDHA in a volume of 200 uL. Initiate reactions with LDHA. Monitor the decrease in NADH absorbance at 340 nm at 37°C for 5 min. Read at Abs340 using Tecan Infinite 1000. Avoid freeze/thaw cycles.
GenBank	NM_005566
Mw(kda)	37.5 kDa

Synonyms	Lactate dehydrogenase A, LDHA
Uniprot	P00338

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Rong Y., et al. Tumour Biol. 2013. 34(3):1523-30. 2. Tang H., et al. Biol Reprod. 2013. 88(4):96.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200567	71081	100 ug	$407		Add to Cart

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