Recombinant human LCK, GenBank Accession No. NM_005356, full length, with N-terminal GST-tag, MW = 83 kDa, expressed in Sf9 insect cells using a baculovirus expression system.
Host cell line: Sf9 cells
Specific activity: 1.96 pmol/min/ug
Immunogen Region
full length
Tag
N-terminal GST-tag
Formulation
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, and 3 mM DTT
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction is conducted at 30°C for 1 hour in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35 and 2 uM substrate using Z-Lyte assay conditions.
Avoid freeze/thaw cycles.
GenBank
NM_005356
Mw(kda)
83 kDa
Synonyms
LCK, Leukocyte C-Terminal Src Kinase, T-Lymphocyte Specific Protein Tyrosine Kinase
Uniprot
P06239
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Fischer, S. et al. Biochem Biophys Res Commun. 1987 Mar 30,143(3):819-26. 2. Veillette, A. et al. Oncogene Res. 1987 Sep-Oct,1(4):357-74.