Human KRAS also known as C-K-RAS, CFC2, and K-RAS2A, GenBank Accession No. NM_033360, a.a. 2-185, with N-terminal His-tag, and expressed in a Baculovirus infected Sf9 cell expression system. MW=22 kDa.
host species: insect
Host species: insect
Host cell line: Sf9 cells
Immunogen Region
2-185
Tag
N-terminal His-Tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, 105 mM Imidazole
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Intrinsic GTPase reaction of KRAS was conducted at room temperature for 60 min using GTPase-Glo (Promega) reagents with 1 uM GTP.
Avoid freeze/thaw cycles.
GenBank
NM_033360
Mw(kda)
22 kDa
Synonyms
C-K-RAS, CFC2, K-RAS2A
Uniprot
P01116
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -70°C.
Reference
1. Roock, W., et al., Annals of Oncology. 2008;19(3):508-515.2. Finocchiaro, G., et al., Journal of Clinical Oncology. 2007 June; 25(18):4021.