Recombinant human KRAS (KRAS proto-oncogene, GTPase), isoform b, encompassing amino acids 2-185. This construct contains an N-terminal His-tag (6xHis). The recombinant protein (Biohippo #11308) was affinity purified and loaded with GDP. Unbound GDP was removed by spin column. Constructs: KRAS (His-2-185) - (GDP)
host species: insect
Host species: insect
Host cell line: Sf9
Immunogen Region
2-185
Tag
N-terminal His-tag
Formulation
20 mM HEPES, pH 7.4, 150 mM NaCl, and 1 mM DTT
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was performed according to KRAS (G12C) Coupled Nucleotide Exchange Assay Kit (Biohippo #78004) with KRAS, Isoform B, His-Tag, GDP-loaded Recombinant (Biohippo #101522) titrated from at various concentrations.