Human KRAS also known as C-K-RAS, CFC2, and K-RAS2A, GenBank Accession No. NM_033360, a.a. 2-185, with G12V mutation and N-terminal His-tag, and expressed in a Baculovirus infected Sf9 cell expression system. MW=22 kDa.
Immunogen Region
2-185
Tag
N-terminal His-Tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, 120 mM Imidazole
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Intrinsic GTPase reaction of KRAS (G12V) was conducted at room temperature for 60 min using GTPase-Glo (Promega) reagents with 1 uM GTP.
GenBank
NM_033360
Mw(kda)
22 kDa
Synonyms
C-K-RAS, CFC2, K-RAS2A
Uniprot
P01116
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -70°C.
Reference
1. Martinelli, P., et al., Gut. 2016;65:476-486.2. Maraver, A., et al., Cancer Cell. 2012 Aug;22(2):222-234.