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Home / Proteins & Peptide

KRAS (G12V), Isoform B, His-Tag, GppNHp-Loaded

SKU : BHP18201071

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Mfr.No.	101359
Description	Recombinant human RAS (GTPase), isoform b, encompassing amino acids 2-185. This construct contains an N-terminal His-tag (6xHis). The protein also contains mutation of interest G12V. The protein (Biohippo #100480) was affinity purified and loaded with GppNHp, a non-hydrolyzable GTP analog. Unbound GppNHp was removed by spin column. Constructs: KRAS (G12V) (His-2-185) - (GppNHp)
Immunogen Region	2-185
Tag	N-terminal His-tag
Formulation	20 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM DTT
Mutation	G12V
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was performed according to KRAS(G12C) Coupled Nucleotide Exchange Assay Kit (SOS1 mediated) (>Biohippo #78004) with various amounts of KRAS (G12V), His-Tag, GppNHp-Loaded. Avoid freeze/thaw cycles.
GenBank	NM_004985
Mw(kda)	22 kDa

Synonyms	GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, N-terminally processed, KRAS, KRAS2, RASK2
Uniprot	P01116

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=90%
Storage	At lease 6 months at -80°C

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201071	101359	4 x 60 ug	$1094.50		Add to Cart

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