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Home / Proteins & Peptide

KRAS (G12D), Isoform A, His-Tag, GppNHp-Loaded Recombinant

SKU : BHP18201083

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BPS Bioscience

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Mfr.No.	101481
Description	Recombinant human KRAS (KRAS proto-oncogene GTPase), isoform a, encompassing amino acids 2-186(end). This construct contains an N-terminal His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease, Biohippo #50308). The protein also contains mutation of interest G12D. The protein was affinity purified and loaded with GppNHp, a non-hydrolyzable GTP analog. Unbound GppNHp was removed by spin column. Ready for use in KRAS-RAF binding studies or inhibitor assays. Constructs: KRAS (G12D) (His-TEV-2-186(end))-(GppNHp) host species: E. coli Host species: E. coli
Immunogen Region	2-186(end)
Tag	N-terminal His-tag
Formulation	20 mM HEPES, pH 7.4, 150 mM NaCl, and 1 mM DTT
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes	Assay was performed according to KRAS(G12C) Coupled Nucleotide Exchange Assay Kit (Biohippo #78004) without SOS1/GTP mixture. KRAS (G12D), Isoform A, His-Tag, GDP-Loaded Recombinant (Biohippo #101312) and GppNHP loaded KRAS (G12D) (Biohippo #101481) were titrated at various concentrations. Avoid freeze/thaw cycles.
GenBank	NM_033360
Mw(kda)	23 kDa

Synonyms	GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, N-terminally processed, KRAS2, RASK2
Uniprot	P01116

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=90%
Storage	At least 6 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201083	101481	4 x 60 ug	$1094.50		Add to Cart

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